<i>Diaporthe</i> species associated with symptomatic and asymptomatic infection of soybean stems in Minnesota: identity, virulence, and growth characteristics

Stem diseases of soybean with symptoms typical infection by Diaporthe spp. are common in the Northern USA. Information is limited, however, on characteristics associated pathogens. This study determined identity and symptomatic asymptomatic stems across diverse environments Minnesota. Plants stem canker, pod blight, plant dieback, as well plants, were sampled. Agar-based media PCR assays used to isolate detect from stems. The isolates identified species based DNA sequencing morphology. plants all environments. Three detected: D. longicolla, caulivora, cucurbitae. longicolla was most widespread distribution. cucurbitae isolated confirmed be a pathogen for first time. Only detected plants. While pathogenic soybean, virulence differed among isolates, cultivars susceptibility. Temperatures 25°C 32°C differentially influenced growth caulivora. All sensitive pyraclostrobin tebuconazol. results advance our understanding different diseases, their management.